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Click Chemistry Tools methyltetrazine (mtz)-peg5-nhs ester 1069-100
Methyltetrazine (Mtz) Peg5 Nhs Ester 1069 100, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methyltetrazine+(mtz/dbco+peg4+biotin/pm39003263-513-0-3
Average 90 stars, based on 1 article reviews
methyltetrazine (mtz)-peg5-nhs ester 1069-100 - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Engineering programmable material-to-cell pathways via synthetic notch receptors to spatially control differentiation in multicellular constructs
Article Snippet: Methyltetrazine (mTz)-PEG5-NHS Ester (Click Chemistry Tools) was first dissolved at 20 mM in DMSO and added to the Fibrinogen solution dropwise to a 0.16 mM final concentration.

Article Title: Stepwise emergence of the neuronal gene expression program in early animal evolution
Article Snippet: Reactions were quenched by adding Tris·HCl pH 7 to a final concentration of 10 mM and methyltetrazine-DBCO (MTZ-DBCO) (Click Chemistry Tools) to 50 μM.

Article Title: Engineering programmable material-to-cell pathways via synthetic notch receptors to spatially control differentiation in multicellular constructs.
Article Snippet: Reactivity was verified by reacting GelMA-mTz at 1% w/v and GFP-TCO at 100 μg/mL for one hour at 37 °C, running on 4–20% SDS-PAGE gel (Bio-Rad) and Coomassie blue staining (Invitrogen).

Article Title: Stepwise emergence of the neuronal gene expression program in early animal evolution
Article Snippet: Barcoding DNA oligonucleotides (Clicktags) with a 5′-amino modifier (Integrated DNA Technologies) were activated by derivatization with methyltetrazine-NHS ester (MTZ-NHS) (Click Chemistry Tools), as originally described.

Article Title: Engineering programmable material-to-cell pathways via synthetic notch receptors to spatially control differentiation in multicellular constructs.
Article Snippet: Methyltetrazine (mTz)-PEG5-NHS Ester (Click Chemistry Tools, 1069-100) was first dissolved at 8.8mM in DMSO and added to GelMA solution dropwise with stirring to 0.88mM final concentration.

Article Title: Engineering programmable material-to-cell pathways via synthetic notch receptors to spatially control differentiation in multicellular constructs
Article Snippet: Methyltetrazine (mTz)-PEG5-NHS Ester (Click Chemistry Tools, 1069-100) was first dissolved at 8.8 mM in DMSO and added to GelMA solution dropwise with stirring to 0.88 mM final concentration.

Incubation:

Article Title: Single-cell joint profiling of multiple epigenetic proteins and gene transcription.
Article Snippet: .. The antibody was then transferred to another tube and mixed with 0.5 μl of 2 mM methyltetrazine (mTz)–PEG4- NHS (Click Chemistry Tools) (the molar ratio of antibody to mTz- PEG4- NHS is 1:10) and incubated at room temperature for 30 min. Two microliters of 50 mM glycine (pH 8.5) was then added to quench any unreacted NHS groups. mTz- labeled antibody was washed twice with 450 μl of 1× BBS as described above and carefully transferred to another tube for reaction with labeled adaptors. ..

Article Title: Single-cell joint profiling of multiple epigenetic proteins and gene transcription
Article Snippet: .. The antibody was then transferred to another tube and mixed with 0.5 μl of 2 mM methyltetrazine (mTz)–PEG4-NHS (Click Chemistry Tools) (the molar ratio of antibody to mTz-PEG4-NHS is 1:10) and incubated at room temperature for 30 min. Two microliters of 50 mM glycine (pH 8.5) was then added to quench any unreacted NHS groups. mTz-labeled antibody was washed twice with 450 μl of 1× BBS as described above and carefully transferred to another tube for reaction with labeled adaptors. ..

Labeling:

Article Title: Single-cell joint profiling of multiple epigenetic proteins and gene transcription.
Article Snippet: .. The antibody was then transferred to another tube and mixed with 0.5 μl of 2 mM methyltetrazine (mTz)–PEG4- NHS (Click Chemistry Tools) (the molar ratio of antibody to mTz- PEG4- NHS is 1:10) and incubated at room temperature for 30 min. Two microliters of 50 mM glycine (pH 8.5) was then added to quench any unreacted NHS groups. mTz- labeled antibody was washed twice with 450 μl of 1× BBS as described above and carefully transferred to another tube for reaction with labeled adaptors. ..

Article Title: Single-cell joint profiling of multiple epigenetic proteins and gene transcription
Article Snippet: .. The antibody was then transferred to another tube and mixed with 0.5 μl of 2 mM methyltetrazine (mTz)–PEG4-NHS (Click Chemistry Tools) (the molar ratio of antibody to mTz-PEG4-NHS is 1:10) and incubated at room temperature for 30 min. Two microliters of 50 mM glycine (pH 8.5) was then added to quench any unreacted NHS groups. mTz-labeled antibody was washed twice with 450 μl of 1× BBS as described above and carefully transferred to another tube for reaction with labeled adaptors. ..



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Journal: Cell

Article Title: Stepwise emergence of the neuronal gene expression program in early animal evolution

doi: 10.1016/j.cell.2023.08.027

Figure Lengend Snippet:

Article Snippet: Barcoding DNA oligonucleotides (Clicktags) with a 5′-amino modifier (Integrated DNA Technologies) were activated by derivatization with methyltetrazine-NHS ester (MTZ-NHS) (Click Chemistry Tools), as originally described.

Techniques: Recombinant, Electron Microscopy, Suspension, Sequencing, Multiplexing, Software, Comparison